Anti-Human Nuclear Antigen Antibody [235-1]

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Flow Cytometry - Anti-Human Nuclear Antigen Antibody [235-1] (A250966) - Antibodies.com

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Immunohistochemistry - Anti-Human Nuclear Antigen Antibody [235-1] (A250966) - Antibodies.com

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SDS-PAGE - Anti-Human Nuclear Antigen Antibody [235-1] (A250966) - Antibodies.com

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Bestseller #A250966

Anti-Human Nuclear Antigen Antibody [235-1]

4 Citations

Product datasheet

5.0 (3 reviews)

Mouse monoclonal [235-1] antibody to Human Nuclear Antigen for Flow Cytometry, IF, IP, ICC and IHC-Fr.

In Stock

£365

Product size:

50µg 100µg

Formats and conjugates:

FITC AP Biotin Cyanine 3 Cyanine 5 Cyanine 5.5 HRP Unconjugated

Request a quotation or Custom or bulk request

Shipping options:

Freight / packing charges: £60 to Germany
Dispatched from: Cambridge (UK)
Lead time: 5-8 business days

Tags:

Trial 10µl Biologically active

Product details

Name: Anti-Human Nuclear Antigen Antibody [235-1]
Description: Mouse monoclonal [235-1] antibody to Human Nuclear Antigen.
Specificity: This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Applications: Flow Cytometry, IF, IP, ICC, IHC-Fr
Dilutions: Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, IP: 1-2 µg / 500 µg protein, ICC: 1-2 µg/ml, IHC-Fr: 1-2 µg/ml
Reactivity: Human, Non-Human Primates
Cross reactivity: This antibody does not cross react with Mouse, Rat, or Chicken.
Immunogen: Nuclei of human myeloid leukemia biopsy cells.

Host: Mouse
Clonality: Monoclonal
Clone ID: 235-1
Isotype: IgG1
Light chains: kappa
Conjugate: Unconjugated
Purification: Protein A/G chromatography.
Concentration: 200 µg/ml
Molecular weight: 70 kDa / 80 kDa
Product form: Liquid
Formulation: Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Scientific validation data (7)

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Immunofluorescence - Anti-Human Nuclear Antigen Antibody [235-1] (A250966)

Immunofluorescent analysis of paraformaldehyde fixed HeLa cells stained with Anti-Human Nuclear Antigen Antibody [235-1] followed by Goat Anti-Mouse IgG (CF® 488) (Green). Counterstain is Phalloidin (Red).

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Flow Cytometry - Anti-Human Nuclear Antigen Antibody [235-1] (A250966)

Flow cytometric analysis of paraformaldehyde fixed HeLa cells using Anti-Human Nuclear Antigen Antibody [235-1] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).

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Immunohistochemistry - Anti-Human Nuclear Antigen Antibody [235-1] (A250966)

Immunohistochemical analysis of acetone-fixed frozen human tonsil using Anti-Human Nuclear Antigen Antibody [235-1].

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SDS-PAGE - Anti-Human Nuclear Antigen Antibody [235-1] (A250966)

SDS-PAGE analysis of Anti-Human Nuclear Antigen Antibody [235-1] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.

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Flow Cytometry - Anti-Human Nuclear Antigen Antibody [235-1] (A250966)

Flow cytometric analysis of PFA fixed Jurkat cells using Anti-Human Nuclear Antigen Antibody [235-1] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).

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Flow Cytometry - Anti-Human Nuclear Antigen Antibody [235-1] (A250966)

Flow cytometric analysis of PFA fixed K562 cells using Anti-Human Nuclear Antigen Antibody [235-1] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).

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Flow Cytometry - Anti-Human Nuclear Antigen Antibody [235-1] (A250966)

Flow cytometric analysis of PFA fixed MCF-7 cells using Anti-Human Nuclear Antigen Antibody [235-1] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).

Product citations (4)

Nature

Reduced neural progenitor cell count and cortical neurogenesis in guinea pigs congenitally infected with Toxoplasma gondii

Species: Pig
Application: Western Blot
Authors: AR Nanna, AV Kel'in, C Theile, JM Pierson, ZX Voo, A Garg, JK Nair, MA Maier, K Fitzgerald, C Rader.

Nature

Reduced neural progenitor cell count and cortical neurogenesis in guinea pigs congenitally infected with Toxoplasma gondii

Species: Pig
Application: Western Blot
Authors: AR Nanna, AV Kel'in, C Theile, JM Pierson, ZX Voo, A Garg, JK Nair, MA Maier, K Fitzgerald, C Rader.

Nature

Reduced neural progenitor cell count and cortical neurogenesis in guinea pigs congenitally infected with Toxoplasma gondii

Species: Pig
Application: Western Blot
Authors: AR Nanna, AV Kel'in, C Theile, JM Pierson, ZX Voo, A Garg, JK Nair, MA Maier, K Fitzgerald, C Rader.

Nature

Reduced neural progenitor cell count and cortical neurogenesis in guinea pigs congenitally infected with Toxoplasma gondii

Species: Pig
Application: Western Blot
Authors: AR Nanna, AV Kel'in, C Theile, JM Pierson, ZX Voo, A Garg, JK Nair, MA Maier, K Fitzgerald, C Rader.

Reviews (3)

Ryan Murray, University of Leeds

June 25, 2024

Application: Western Blot, Immunofluorescence
Species: Human
Sample: Human aortic endothelial cells (HAECs)
Experimental
conditions: WB: 4-20% gel, PVDF membrane, 1:3000 primary. IF: 4% PFA fixation, 0.1% Triton-X permeabilization, primary 1:300, secondary 1:300.
Dilution: WB: 1:3000, IF: 1:300
Results summary: Observed 95kDa band (CRACR2A-L). High specificity, low background, high sensitivity, reproducible.
Comments: Minimal background, clear clustering, strong signal.

Ryan Murray, University of Leeds

June 25, 2024

Application: Western Blot, Immunofluorescence
Species: Human
Sample: Human aortic endothelial cells (HAECs)
Experimental
conditions: WB: 4-20% gel, PVDF membrane, 1:3000 primary. IF: 4% PFA fixation, 0.1% Triton-X permeabilization, primary 1:300, secondary 1:300.
Dilution: WB: 1:3000, IF: 1:300
Results summary: Observed 95kDa band (CRACR2A-L). High specificity, low background, high sensitivity, reproducible.
Comments: Minimal background, clear clustering, strong signal.

Ryan Murray, University of Leeds

June 25, 2024

Application: Western Blot, Immunofluorescence
Species: Human
Sample: Human aortic endothelial cells (HAECs)
Experimental
conditions: WB: 4-20% gel, PVDF membrane, 1:3000 primary. IF: 4% PFA fixation, 0.1% Triton-X permeabilization, primary 1:300, secondary 1:300.
Dilution: WB: 1:3000, IF: 1:300
Results summary: Observed 95kDa band (CRACR2A-L). High specificity, low background, high sensitivity, reproducible.
Comments: Minimal background, clear clustering, strong signal.